Johns Hopkins University School of Medicine alumnus and former faculty member Hamilton O. Smith, whose 1978 Nobel Prize-winning discovery of restriction enzymes revolutionized genetic engineering, ...

A Nobel laureate, he identified an enzyme that cuts DNA, laying the groundwork for milestones in scientific research and ...

Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research; however, they have limited sequence specificity and availability. The ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

Research by Professor of Chemical and Biomolecular Engineering Huimin Zhao and graduate student Behnam Enghiad at the University of Illinois is pioneering a new method of genetic engineering for basic ...

TALENs involve the synthesis of the C-terminal with type II restriction enzyme, Fokl, in order to produce heterodimers that form a double-stranded break (DSB) in DNA. Recent advancements in TALEN ...

Scientists are pioneering a new method of genetic engineering for basic and applied biological research and medicine. Their work has the potential to open new doors in genomic research by improving ...